IV Congreso Internacional de Ciencia y Tecnología de Alimentos - Córdoba, Argentina 14 a 16 de Noviembre de 2012
262
Figure 1: D. tertiolecta (a) Autotrophic cultivation (b) Mixotrophic cultivation; T. suecica (c) Autotrophic
cultivation (d) Mixotrophic cultivation () Biomass (g L
-1
); (▪) pH; () Glycerol (g L
-1
)
The growth curves of Figure 1 shows an increase in maximum concentration of biomass of microalgae D.
tertiolecta in mixotrophic cultivation, reaching 0.29 g L
-1
at 10
th
day, while the autotrophic cultivation was
0.25 g L
-1
on day 12. The growth of microalgae T. suecica in autotrophic and mixotrophic cultivation did not
present a statistically significant difference, obtaining maximum biomass concentration of 0.43 g L
-1
on the
9
th
day and 0.41g L
-1
, on day 6, respectively.
The consumption of glycerol by D. tertiolecta was 18.2% while T. suecica corresponded to 24.8%.
Table 2 shows a comparison of autotrophic and mixotrophic cultivation of microalgae D. tertiolecta and T.
suecica in relation to growth parameters evaluated.
Table 2: Mean values ± standard deviation for maximum biomass concentration, productivity and maximum
specific growth rate of microalgae D. tertiolecta and T. suecica and statistical analysis of the data
Parameters (mean
± standart
deviation)
Dt – Dunaliella tertiolecta; Ts – Tetraselmis suecica; X
max
– Maximum biomass concentration; P –
Productivity; μ
max
– Maximum specific growth rate.
*
Equal small letters indicate that there is no meaning difference between lines, at 95% of confidence
(p<0.05).
Note that the mixotrophic cultivation of D. tertiolecta and T. suecica also contributed in increasing
productivity and maximum specific growth rate. Thus, it was observed that the addition of glycerol to the
culture medium contributed positively in the increment of the parameters studied, with emphasis on
microalgae T. suecica.
According to Garcia et al. (2000) increased biomass possibly associated with this synergistic effect of light
and organic substrate (glycerol).
Studies reporting the mixotrophic cultivation D. tertiolecta and T. suecica are not found in the literature.
0 1 2 3 4 5 6 7 8 9 10 11 12
Time (Days)
0,00
0,05
0,10
0,15
0,20
0,25
0,30
0,35
Biomass (g L
-1
)
6,0
6,5
7,0
7,5
8,0
8,5
9,0
9,5
pH
0 1 2 3 4 5 6 7 8 9 10
Time (Days)
0,00
0,05
0,10
0,15
0,20
0,25
0,30
0,35
0,40
0,45
0,50
Biomass (g L
-1
)
7,0
7,2
7,4
7,6
7,8
8,0
8,2
8,4
8,6
8,8
9,0
9,2
pH
0 1 2 3 4 5 6 7 8 9 10 11 12
Time (Days)
0,00
0,05
0,10
0,15
0,20
0,25
0,30
0,35
Biomass (g L
-1
)
3
4
5
6
7
8
9
pH
Glycerol (g L
-1
)
0 1 2 3 4 5 6 7 8 9 10
Time (Days)
0,0
0,1
0,2
0,3
0,4
0,5
Biomass (g L
-1
)
3
4
5
6
7
8
9
pH
Glycerol (g L
-1
)
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