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Air Naturel GOTA Ultrasonic Air Humidifier User Manual Page 236

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IV Congreso Internacional de Ciencia y Tecnología de Alimentos - Córdoba, Argentina 14 a 16 de Noviembre de 2012
226
For the verification of the model, regression coefficients were calculated and ANOVA performed. Results
are shown in Table 4. The terms that were not significant were added to the lack of fit to calculate the
analysis of variance.
Table 4: Analysis of variance for the central composite rotational design 23 for the total reducing sugar
Source of
variation
Sum of
squares
Degrees of
freedom
Mean
square
p -
value
Regression
3728,12
2
1864,06
6,68
Rresidue
3625,88
13
278,91
Total
7254,0
15
Total reducing sugar (R: 0,71, P
2; 13 0,95
= 3,81).
The correlation coefficients (R): 0.71 was calculated by ANOVA (Table 4) for the total reducing sugar;
therefore, it can be concluded that the model fitted well to the experimental data. The p-value/p-table ratio
was 1.7 for total reducing sugar, making the model predictive and meaningful for the response studied in this
range.
Thus it was possible to generate the model equation with the coded variables representing the responses of
the RCCD as a function of variables percentage of earth, temperature and contact time, as shown in Equation
1.
ART (g.L
-1
): 60 -14,78.X
2
² + 10,84.X
1
.X
2 (1)
(1)
where: X
1
: percent diatomaceous earth; X
2
: Temperature; X
3
: Contact time with the substrate.
Figure 1: Contour curves for total reducing sugar response (A
1
, B
2
, and A
3
) for pre-treated molasses.
Using the model it was possible to construct contour curves (Figure 1), obtaining concentrations of the
variables: the percentage of earth, temperature and time of contact with the substrate.
With the contour curves shown in Figure 1 it is verified that to keep a high sugar rate on the substrate one
can work with a low temperature 60 to 64°C and percentage of earth of 6 to 7% or high temperatures 76-
80°C with earth percentage of 7-8% (Figure 1 A
1
). Percentage of earth should be 6-7% with a contact time
from 1.33 to 1.5 h, or one can work at high earth percentage from 7 to 8% and contact time from 2 to 2.17h
(Figure 1 A
2
).
In the use of a temperature of 60°C one should work with time of 1.33 h, or high temperature of 80°C with a
time of 2.17 hours (Figure 1 A
3
). Therefore the best condition for the sugars present in the substrate must
work with 6-7% diatomaceous earth at 60°C and contact time from 1.33 to 1.5 h under stirring at 150 rpm in
a reactor (300 mL) cased to remove heavy metals and possibly increase various types of bioproduction when
working with this type of coproducts. According to Machado (2013) who used Sporidiobolus
pararoseus for production of carotenoids in medium formulated corn steep liquor (6.5 g.L
-1
) and
sugar cane molasses (40 g.L
-1
) pre-treated with these coproducts land diatomaceous obtained a gain
of approximately 9% increase in production in carotenoids (570.95 µ.L
-1
), besides an increase in
biomass of 20% (9.43 g.L
-1
) when compared to the culture medium without prior treatment.
60
50
40
30
20
10
0
5,32 6 7 8 8,68
Percentage of land
60
64
70
76
80
Temperature (°C)
60
50
40
30
20
10
0
5,32 6 7 8 8,68
percentage of land
1,33
1,5
1,75
2
2,17
Time (h)
90
80
70
60
50
40
60 64 70 76 80
Temperature (°C)
1,33
1,5
1,75
2
2,17
Time (h)
A
1
)
A
2
)
A
3
)
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